Extended Data Fig. 4: Contrast and resolution is enhanced after multiview fusion or SIM.

a) Nanoscale imaging of organelles (mitochondrial outer membrane, cyan; and double stranded DNA, magenta) in expanded U2OS cells, to accompany Fig. 1f. Top: images, bottom: line profiles corresponding to a1–a3 at top. b) Neurons marked with membrane targeted GFP in living C. elegans embryos, to accompany Fig. 2i. Top: images; bottom: line profiles corresponding to b1–b4 at top, n = 5 profiles at indicated positions were used to generate means ± standard deviations for b1, b2. c) Stained actin in fixed B16F10 mouse melanoma cells embedded in collagen gels, to accompany Fig. 3c. Left: images; right: line profiles corresponding to c1–c4 at left. d) Neurons in fixed, expanded C. elegans embryos, to accompany Fig. 3e, f. Top: images; bottom: line profiles corresponding to d1–d6 at left, n = 7 profiles at indicated positions were used to generate means ± standard deviations for d1–d3. e) histone H2B puncta in live Jurkat T cells, to accompany Fig. 4c. Top: images; bottom: line profiles corresponding to e1–e3 at top, n = 10 profiles at indicated positions were used to generate means ± standard deviations for e1–e3. Scale bars: a, d) 2 µm, b, c, e) 5 µm.