Extended Data Fig. 5: Triple-view comparisons in adult C. elegans.

a) Axial views of whole fixed worm labelled with NucSpot Live 488, comparing raw views gathered by objectives A, B, C; triple-view reconstruction; and point-scanning confocal microscope. Arrows highlight example region with uniformly good quality in triple-view reconstruction, but showing attenuation either at bottom (Views A, B) or top (View C, point-scanning confocal) of stack with other methods. See also Fig. 2g–j. b) Average attenuation measured through axial extent of the worm, as measured by raw views A, B C (top graph) and triple-view reconstruction and point-scanning confocal (bottom graph). Exponential fits to the data are also shown with dashed lines. See also Supplementary Methods. c–f) Comparative higher magnification views of dashed red rectangular region in a), with bottom deconvolved view c), commercial Leica SP8 confocal microscope e), conventional triple-view deconvolution d), attenuation-compensated triple-view deconvolution f). Coloured arrows highlight comparisons, orange: single-view versus triple-view, magenta: deconvolution methods. Scale bar: a) 50 μm, c–f) 10 μm.