Extended Data Fig. 2: Analysis of RBD-specific memory B cells.
From: Immunogenicity and efficacy of heterologous ChAdOx1–BNT162b2 vaccination
(A) Gating strategy. From left to right, the first three pseudo-color plots show the successive gates applied for single cells and viable cells. B cells were gated as CD19+/CD3- cells (4th plot). Within the B cell gate, memory B cells (mBCs) were defined as non-naïve B cells on the same biparameter plot (5th plot). (B-E) Concatenated phenotypic profiles of RBD-specific mBCs for all individuals in the ChAd/BNT and BNT/BNT groups at three time points (before vaccination, before the 2nd dose, after the second dose). For each group, the phenotypic profiles correspond to the concatenation of the FACS analysis data collected from 30 individual vaccinees. (B) Visualization of RBD-binding B cells in the mBC gate. (C) Visualization of the proportion of switched (IgD-) and unswitched (IgD+) B cells among the RBD-specific mBC pool. (D) Visualization of the pattern of Ig isotype expression by RBD-specific mBCs. (E) Visualization of the distribution of RBD-binding B cells within the resting (CD21+CD11c-) and “activated” (CD21-CD11c+/CD21-CD11c-/ CD21+CD11c+) mBC compartments. The mean frequencies of RBD-specific mBCs falling into each quadrant are indicated in red.
