Extended Data Fig. 9: CX3CR1+ cells were an important source of IL-27 during HFD-induced obesity.
From: IL-27 signalling promotes adipocyte thermogenesis and energy expenditure
a–c. Lyz2-Cre Il27p28f/f and Il27p28f/f littermates at 8 weeks of age were treated with high fat diet (HFD) for 10 weeks. a. Body weight changes were recorded each week (n = 9 for Il27p28f/f and n = 6 for Lyz2-Cre Il27p28f/f). Intraperitoneal GTT (b) and ITT (c) were performed after 10 weeks of treatment (n = 7 for Il27p28f/f and n = 6 for Lyz2-Cre Il27p28f/f). d–g. Itgax-Cre Il27p28f/f and Il27p28f/f littermates at 8 weeks of age were fed on HFD for 10 weeks. Body weight changes were recorded each week (d, n = 8 for Il27p28f/f and n = 15 for Itgax-Cre Il27p28f/f). Intraperitoneal GTT (e, n = 8 for Il27p28f/f and n = 15 for Itgax-Cre Il27p28f/f) and ITT (f, n = 6) was performed after 10 weeks of treatment. g. Adipose tissues were collected and weighted after 10 weeks of HFD treatment (n = 8 for Il27p28f/f and n = 15 for Itgax-Cre Il27p28f/f). h&i. Adipoq-Cre Il27p28f/f and Il27p28f/f littermates at 8 weeks of age were fed on HFD for 10 weeks. h. Body weight was recorded each week (n = 6). i. Intraperitoneal GTT was performed after 10 weeks of treatment (n = 6). j–l. Cx3cr1-Cre Il27p28f/f and Il27p28f/f littermates at 8 weeks of age were fed on HFD for 10 weeks. j. Body weight was recorded each week (n = 8). Intraperitoneal GTT (k) and ITT (l) were performed after 10 weeks of treatment (n = 8). All experiments were repeated at least twice with similar results. Data are mean ± s.e.m. of biologically independent samples. Two-way ANOVA (a, d & h); two-way ANOVA with Sidak’s multiple comparisons test (b, c, e, f & i–l); two-tailed unpaired student’s t-test (g).
