Extended Data Fig. 8: Effect of ILC3-specific MHCII and generalized intestinal inflammation on neuroinflammation.

a-d, Naive 2D2 T cells (Thy1.1⁺) were transferred into recipient mice, which were immunized 24 h later to induce active EAE. At day 14 EAE, frequencies and counts (a), activation/proliferation (b), and polarization (c) of donor Thy1.1⁺ 2D2 T cells (c, upper panel) or endogenous T cells (c, lower panel) were analysed by intracellular flow cytometry in the cLN or inLN (n = 9 mice/group (a, b), n = 5 mice/group (c)). d, Frequencies of IFNγ-producing endogenous T cells in the CNS were quantified by intracellular flow cytometry cytokine staining (n = 8 mice/group). e-f, Passive EAE was induced in recipient mice and day 15 frequencies of Thy1.1⁺ 2D2 T cells in the cLN and CNS were determined by flow cytometry (n = 4 mice/group) (e). Representative H&E of fixed transverse spinal cord sections of mice shown at 4X magnification (top, scale bar = 300 µM) with 20X inset magnification (bottom, scale bar = 75 µm) of cellular infiltration near ventromedial fissure at day 15 passive EAE (f). g-h, Average clinical scores in indicated mice (MHCII^ΔTcell = CD4-cre⁺ x H2-Ab1^fl/fl) after induction of active EAE (n = 4 mice/group (g), n = 5 mice/group (h)). i, C57BL/6 mice were treated with either 3% D.S.S. in the drinking water for 7 days or were orally gavaged with C. rodentium (n = 5 mice/group). Active EAE was induced 14 days later and clinical scores were taken on mice or controls. Data are representative of two (g-i) or three (a-f) independent experiments with similar results. Data are pooled from two independent experiments in a, b, d. Results are shown as mean ± s.d. (a-e) or s.e.m (g-i) Statistics are calculated by two-way analysis of variance (ANOVA) (c, d) with Sidak’s multiple comparisons test or unpaired, two-tailed t-test (Mann–Whitney U-test) (a, b, e).

Source data