Extended Data Fig. 2: Phenotypic analyses of ILC3s in the CNS and peripheral blood.
From: Antigen-presenting innate lymphoid cells orchestrate neuroinflammation
a-c, Heat maps showing absolute log normalized counts (one plus log 2) (a, b) or relative expression Z-scores (c) from RNA sequencing of indicated lineage-specifying genes in sorted ILC3s from the cLN, CNS, or small intestine lamina propria (SI-LP) of Rorc-eGFP mice during peak of active EAE (n = 4 mice). d, Quantification of cytokine production by ILC3s (CD45+, CD3ε-, CD5-, CD8α-, TCRγδ-, NK1.1-, CD11b-, CD11c-, B220-, CD127+, CD90.2+, KLRG1-, RORγt+) in indicated tissues during EAE (day 15) C57BL/6 mice (n = 4 mice). e, Representative flow cytometry gating strategy to detect ILC3s in human PBMCs (Lineage = CD19, CD94, CD14, CD123, FcR1a, CD11c) and frequency quantification of ILC3s in healthy control (HC) or RRMS samples (n = 18 sample-pairs – Supplementary Table 1). Data in d are representative of two independent experiments with similar results. Data in e are pooled and representative of two independent flow cytometry experiments on cryopreserved PBMC sample sets with similar results. Results are shown as mean ± s.d. Statistics are calculated by one-way analysis of variance (ANOVA) with Sidak’s multiple comparisons test (d).
