Extended Data Fig. 5: ILC3s are found in the cerebral spinal fluid of patients with RRMS and express HLA-DR and CD86.
From: Antigen-presenting innate lymphoid cells orchestrate neuroinflammation
a, Validation of anti-RORγt antibody staining on single cell suspensions from human donor tonsils compared to FMO (Fluorescence Minus One) control indicating staining without anti-RORγt antibody. b-e, Cerebral spinal fluid (CSF) was obtained from 7 patients with RRMS and one control individual (other neurological disease, OND). Individuals were further stratified by the presence or absence of contrast enhancing lesions (CEL) as well as CEL number (Supplementary Table 2). CSF was processed immediately by centrifuging for 10 min at 400 x g and staining for ILC3s as indicated (b) (Lineage = CD19, CD94, CD14, CD123, FcR1a, CD11c). Gated RORγt+ populations in the indicated tissues exhibited dim staining for CD45, which is a defining feature of ILC3s, relative to CD4 T cells (c). Indicated frequencies of ILC3s in the CSF were quantified (d). PBMCs were used as controls during each collection and used for comparison of HLA-DR or CD86 expression on ILC3s in the CSF (e). Results are shown as mean (d) and RRMS flow cytometry is representative flow cytometry from CSF of patient #2 (Supplementary Table 2). Human tonsil data is representative of 3 individual tonsil samples.
