Extended Data Fig. 5: aDCN activity suppresses food intake regardless of hedonic value of food.
From: Reverse-translational identification of a cerebellar satiation network
(a) Experimental timeline: 24-h food deprivation followed by measurements of chow intake over 12-h (top). Cumulative kcal of chow intake in food-deprived mice with hM3D(Gq) expression in the aDCN-LAT following vehicle or CNO treatment (bottom; n = 9 hM3D(Gq); repeated measures two-way ANOVA interaction P < 0.001, main effect P < 0.001; Holm-Sidak’s, P = 0.094 (30 min), P=0.008 (1 h), P < 0.001 (2 h), P < 0.001 (4h), P < 0.001 (6h), P < 0.001 (8 h), P<0.001 (10 h), P < 0.001 (12 h)). (b) 12-h food intake in food-deprived mice expressing hM3D(Gq) in the aDCN-LAT (n = 9 mice, paired t-test, P = 0.008). (c) 12-h food intake in food-deprived mice expressing mCherry in the aDCN-LAT (n = 8 mice, paired t-test, P = 0.391). (d) Food intake during the first 2-h of refeeding in food-deprived mice expressing hM3D(Gq) in the aDCN-LAT (n = 9 mice, paired t-test, P < 0.001). (e) Food intake during the first 2-h of refeeding in food-deprived mice expressing mCherry in the aDCN-LAT (n = 8 mice, paired t-test, P = 0.223). (f) Experimental timeline: 24-h food deprivation followed by measurement of high fat high sugar (HFHS) diet intake over 12 h (top). Cumulative kcal of HFHS diet intake in food-deprived mice with hM3D(Gq) expression in the aDCN-LAT following vehicle or CNO treatment (bottom; n = 9 hM3D(Gq) mice; two-way repeated measures ANOVA interaction P < 0.001, main effect P<0.001; Holm-Sidak’s, P < 0.001 (30-min), P < 0.001 (1-h), P<0.001 (2-h), P < 0.001 (4-h), P<0.001 (6-h), P < 0.001 (8-h), P<0.001 (10-h), P < 0.001 (12-h)). (g) 12-h HFHS diet intake in food-deprived mice expressing hM3D(Gq) in the aDCN-LAT (n = 9 mice, paired t-test, P < 0.001). (h) 12-h HFHS diet intake in food-deprived mice expressing mCherry in the aDCN-LAT (n = 8 mice, paired t-test, P = 0.527). (i) Calorie intake during the first 2-h of HFHS diet refeeding in food-deprived mice expressing hM3D(Gq) in the aDCN-LAT (n = 9 mice, paired t-test, P < 0.001). (j) Calorie intake during the first 2-h of HFHS diet refeeding in food-deprived mice expressing mCherry in the aDCN-LAT (n=9 mice, paired t-test, P = 0.686). Data are expressed as mean ± SEM, two-sided P values, t-tests and post-hoc comparisons: **P<0.01, ***P < 0.001, ANOVA interaction: ∞∞∞P<0.001; ANOVA main effect of group: ¤¤¤P < 0.001. Statistical analysis in Supplementary Table 1
