Extended Data Fig. 9: Metabolite impacts on SCWG.
From: Gut microbiota modulates weight gain in mice after discontinued smoke exposure
(a–b) Weight change during smoke exposure and cessation upon addition of N-formylanthranilic acid (a, N-FAN acid) or trigonelline (b, TRI). a: n = 10 HFD-fed mice per group, mean ± s.e.m; day 42: unpaired 2-sided t test; iAUC: unpaired 2-sided t test, mean ± s.e.m. b: Non-SMK+abx+PBS, Non-SMK+abx+TRI and SMK+abx+TRI (n = 10), SMK+abx+PBS (n = 9), HFD-fed mice, mean ± s.e.m; day 35: one-way ANOVA Sidak correction; inset: iAUC; one-way ANOVA Sidak correction, mean ± s.e.m. (c) iAUC of weight change at smoke exposure or cessation of Fig 4a. NS+abx (n = 9), SMK+abx+PBS (n = 10 smoke exposure, n = 9 cessation), all other groups n = 10; one-way ANOVA Sidak correction; box plots represent max-min values. Rx- treatment. (d) Plasma levels of metabolites of the DMG biosynthesis pathway assessed by targeted mass spectrometry in mice consuming choline-deficient diet (CDD), n = 10; two-sided Mann-Whitney U-test, mean values. (e) Hepatic BHMT qPCR during smoke exposure in mice consuming CDD, n = 10; unpaired two-sided t-test, mean values. (f) Targeted mass spectrometry of plasma DMG. PBS (n = 9), DMG (n = 10) HFD-fed mice; two-sided Mann-Whitney U-test, mean values. (g–h) Weight change during DMG supplementation on day 14 (g, n = 10) or day 49 (h, PBS: n = 19, DMG: n = 18, pool of 2 independent repeats), mean ± s.e.m., HFD-fed mice; last time point: unpaired two-sided t-test. Inset: iAUC, unpaired two-sided t-test, mean ± s.e.m. (i) Plasma levels of HFD-fed mice treated with PBS (n = 8 for all paraments except n = 7 for NH3) or DMG (n = 10 for all parameters except n = 9 for ALT); unpaired two-sided t-test, except two-sided Mann Whitney U test for NH3 and HDL; mean values. (j–m) Metabolic cage analysis for 172h in DMG versus PBS supplemented mice. Locomotion (j, n = 8), respiratory exchange ratio (k, RER, n = 4), energy expenditure (l, n = 4) and food intake (Total kcal, m, n = 8). Inset: AUC; unpaired two-sided t-test, mean ± s.e.m. Grey background depicts the cessation period (a–c) or the dark cycle (j–i). *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001, P values presented in Supplementary Table 1.
