Extended Data Fig. 10: ACG supplementation ameliorates weight gain.
From: Gut microbiota modulates weight gain in mice after discontinued smoke exposure
(a) Plasma levels of ACG during smoke exposure (day 15) and cessation (day 30). Non-SMK (n = 7 for smoke exposure, n = 5 for cessation), SMK (n = 6 for smoke exposure, n = 5 for cessation), Non-SMK+abx (n = 8 for smoke exposure, n = 6 for cessation), SMK+abx (n = 7 for smoke exposure, n = 6 for cessation), HFD-fed mice; two-way ANOVA and BH correction (Q < 0.1), mean values. (b) Calories measured in rodent diet. n = 3 mice per group; one-way ANOVA and Tukey correction, mean values. (c) Plasma levels of ACG in HFD (Control, n = 10) or HFD+ACG (ACG, n = 9) mice, assessed by targeted mass spectrometry. Unpaired two-sided t-test, mean values. (d) Weight change during ACG supplementation. n = 5 HFD-fed mice per group, mean ± s.e.m.; day 14: unpaired two-sided t-test. Inset: iAUC, unpaired two-sided t-test, mean ± s.e.m. (e) GTT during ACG administration (day 49) n = 10 HFD-fed mice per group. Inset: AUC, Unpaired two-sided t-test, mean ± s.e.m. (f) Plasma biochemical levels Control (n = 19 except n = 17 for TG & urea, n = 18 for albumin). ACG (n = 19 except for n = 18 for NH3) HFD-fed mice. Two-sided Mann-Whitney U-test, except for unpaired two-sided t-test for glucose, total cholesterol, urea and total protein, mean values. (g–j) Metabolic cage analysis for 384h, n = 4 HFD-fed mice per group, dashed line indicates the starting day of the diet. (g) Food intake (total kcal); (h) locomotion (‘cnts’ refers to light beam break count); (i) respiratory exchange ratio (RER); and (j) energy expenditure; inset: AUC, unpaired two-sided t-test except two-sided Mann-Whitney U-test for energy expenditure, mean ± s.e.m. Grey background depicts the dark cycle. (k) Plasma DMG levels in Control (n = 7) or ACG (n = 6) mice, unpaired two-sided t-test, mean values. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001, exact P values presented in Supplementary Table 1.
