Extended Data Fig. 13: Potential microbiome and metabolite associations with human who smoke.
From: Gut microbiota modulates weight gain in mice after discontinued smoke exposure
(a) Experimental outline of the human cohort. (b–f) human fecal microbiome analysis (Non-SMK n = 40, SMK n = 20). (b) PCA of metagenomically-assembled genomes (MAGs) relative abundances in human stool; inset: PERMANOVA. (c) Differentially abundant MAGs; asterisks denote significant differences (p<0.05); two-sided Mann-Whitney U-test. (d) PCA of KO annotated reads; inset: PERMANOVA. (e) KEGG orthologs of highest effect (feature with negative values enriched in SMK), two-sided Mann-Whitney U-test, highest (and lowest), 2-fold log change. (f) ROC curves for binary classifier (methods). (g–i) Targeted mass spectrometry of metabolites from the DMG biosynthesis pathway: choline (g), betaine (h) and DMG (i); Non-SMK (n = 62), SMK (n = 34); two-sided Mann-Whitney U-test, mean values. *P<0.05; **P<0.01, exact P values presented in Supplementary Table 1, raw data presented in Supplementary Table 7.
