Extended Data Fig. 5: Nicotine impacts on the microbiome and SCWG.
From: Gut microbiota modulates weight gain in mice after discontinued smoke exposure
(a) Nicotine and cotinine levels in peripheral plasma, portal vein plasma and stool in naive SPF and GF mice. SPF (n = 6), GF (n = 5 for plasma and portal vein, n = 6 for stool); two-sided Mann-Whitney U test, mean values. (b) Plasma nicotine and cotinine levels during smoke exposure. Non-SMK (n = 8), SMK and SMK+abx (n = 10), Non-SMK+abx (n = 9), HFD-fed mice, Kruskal-Wallis and Dunn’s test, mean values. (c) Fecal nicotine levels in HFD-fed mice exposed for 3 weeks to cigarettes smoke or to nicotine by intra-peritoneal (i.p.) injections; n = 10 mice per group; Kruskal-Wallis and Dunn’s test, mean values. (d) PCoA depicting Jaccard similarity in mice exposed to cigarette smoke or to i.p. nicotine; Naive (n = 9), SMK (n = 10), nicotine-administered (i.p., n = 8) mice; pairwise PERMANOVA. (e) Weight change in mice exposed to cigarette smoke or to i.p. nicotine; Naïve and nicotine (n = 9), SMK (n = 10); day 21: one-way ANOVA and Tukey correction, mean values. (f–g) 16S rDNA-based PCoA depicting (f) Bray-Curtis dissimilarity and (g) Jaccard distance; Naive and SMK (n = 10), nicotine-administered via drinking water (n = 9), pairwise PERMANOVA. (h) Weight change in mice exposed for 3 weeks to cigarette smoke or to nicotine via drinking water. Naive and SMK (n = 10) nicotine (n = 9); day 21: one-way ANOVA and Tukey correction, mean values. (i) Plasma levels of nicotine and cotinine in HFD-fed mice administered with PBS or nicotine by osmotic pumps for 3 weeks, n = 10 mice per group; two-sided Mann-Whitney U-test, mean values. (j) Weight change in HFD-fed mice administered with PBS or nicotine by osmotic pumps for 4 weeks. n = 10 mice per group; day 28: unpaired two-sided t-test, mean values. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001, P values presented in Supplementary Table 1.
