Extended Data Fig. 1: TDP-43 pathology of ALS with FTLD continued.
From: Structure of pathological TDP-43 filaments from ALS with FTLD
a, Staining of TDP-43 (brown) in the frontal and motor cortices and spinal cords of individuals 1 and 2 with anti-TDP-43 (amino acids 203–209) antibody. Nuclei were counterstained in blue. Scale bars, 50 μm. b, Staining of TDP-43 glial cytoplasmic inclusions (brown) in the motor cortices of individuals 1 and 2 with anti-phosphorylated S409 and S410 TDP-43 antibody. Nuclei were counterstained in blue. Scale bars, 50 μm. c, d, Immunoblots of the total homogenate (T), Sarkosyl-soluble fraction (S) and Sarkosyl-insoluble fraction (I) from the motor cortices of individuals 1 and 2 with anti-TDP-43 (amino acids 203–209) (c) and anti-phosphorylated S409 and S410 TDP-43 (d) antibodies. The original, uncropped blots are shown in Supplementary Fig. 1. e, Immuno-electron microscopy of the Sarkosyl-insoluble fraction from the frontal cortex of individual 2 using anti-phosphorylated S409 and S410 TDP-43 antibody. Scale bar, 100 nm. a–e, Similar results were obtained in at least three independent experiments.
