Extended Data Fig. 6: Results of in vivo modelling of clonal competition.

Proportional bubble plot of Barcode-seq data showing dominant clones in the Competition 1:1:1 (a) and Competition 80:15:5 (b) groups at Baseline and Disease timepoints. Red, green and blue regions show MLL-AF9 + Flt3^ITD, MLL-AF9 and MLL-AF9 + Kras^G12D derived clones respectively (n = 4 mice per group) c, Correlation plots of clonal repertoires of MLL-AF9 + Kras^G12D clones from baseline and disease samples in the Competition 1:1:1 (left) or Competition 80:15:5 (right) groups (n = 4 mice per group) d, Venn diagram indicating the number of shared and distinct MLL-AF9 + Kras^G12D clones at Disease between Kras only, Competition 1:1:1 and Competition 80:15:5 groups. e, Clone size at Baseline (T₀) expressed as log₁₀ transformed counts per million (CPM) of Competition 80:15:5 specific clones (n = 22 clones) vs Kras specific clones (n = 22 clones). Boxplots span the upper quartile (upper limit), median (centre) and lower quartile (lower limit). Whiskers extend a maximum of 1.5x IQR. One-sided T-test (*, p = 0.018). f, Representative flow cytometry analysis of the disease burden in bone marrow and spleen 2 weeks after equal numbers of cells from each genotype were injected into separate mice. Plots are representative of 1 mouse per group. g, Proportional bubble plot of normalized Barcode-seq data showing clonal repertoire of Kras clones from Baseline samples in the Competition 1:1:1, Competition 80:15:5 or MLL-AF9 + Kras^G12D only groups. h, Correlation plots of the barcode repertoires present in Baseline cells sampled from the MLL-AF9 + Kras^G12D only transplants and the Competition 1:1:1 and Competition 80:15:5 transplants. Inset values indicate adjusted R² and p-values using a one-tailed F-test for linear regression on CPM of barcodes detected at both timepoints. Results in this figure are representative of n = 4 mice per experimental group.