Extended Data Fig. 4: Generation and genetic characterization of secondary and tertiary leukaemic cells.
From: Non-genetic determinants of malignant clonal fitness at single-cell resolution
a, Schematic of a SPLINTR experiment using 2° MLL-AF9 cells. Timepoints where whole exome sequencing (WES) and low-coverage whole genome sequencing (LC-WGS) analysis was conducted are indicated. b, Results of whole exome sequencing (WES) of SPLINTR barcoded tertiary MLL-AF9 leukaemic cells at Endpoint (TN), expressed as mutations per Megabase (1 million base pairs). Points indicate raw result from individual mice (n = 2). Lines indicate the mean. Full lists of variants identified can be found in Extended Data Table 3. c, Copy number variant profiles from LC-WGS data of SPLINTR barcoded tertiary MLL-AF9 leukaemic cells from bone marrow at Endpoint (top) and a sex and age matched healthy wild type C57BL/6 littermate control (bottom). d, UMAP projection of reanalysed MLL-AF9 + KrasG12D Baseline scRNA-seq data from Fig. 2. Cells comprising Shared dominant clones are highlighted (top) and normalized expression level of transcripts derived from the MLL-AF9 retroviral construct (bottom).
