Extended Data Fig. 6: Rac during durotaxis.
From: Collective durotaxis along a self-generated stiffness gradient in vivo
a–b, Cells at the front and rear of neural crest explants on a stiffness gradient, with Rac-GTP, Phalloidin and DAPI labelling (a), and quantification of Rac-GTP along the axis from the edge of the cluster inwards (b). Note that Rac-GTP preferentially accumulates at the cell edge irrespective of its position within the cluster, consistent with previous observations of Rac polarity. Scale bar is 10 μm (a). c, d, Immunostaining of vinculin with Phalloidin in explants on uniform stiffness (c, top; m) or a physiological stiffness gradient (c, bottom) and polarity of the number of vinculin spots quantified (d). Scale bar is 5 μm (c). e, f, Immunostaining of Rac-GTP in control or Integrin-β1 knockdown (e) and quantification of its polarity (f). Scale bar is 50 μm (e). Thick bars (b, d, f) represent mean; error bars (b, d, f) represent s.d.; two-tailed Mann-Whitney U test (d, f), ****P≤0.0001; n = 20 (b, d, f) clusters. Statistics and reproducibility are in the source data and Methods
