Extended Data Fig. 7: Durotaxis and chemotaxis cooperatively coordinate neural crest migration in vivo.
From: Collective durotaxis along a self-generated stiffness gradient in vivo
a, Schematics indicating the different treatments. Results are shown in Fig. 4a, b. b, Stiffness measurements in control and ablated embryos. Quantification of in vivo migration in each condition is shown in Fig 5b. c, d, An example heat map of stiffness from local pressure treatment (c) as depicted in Fig. 4c and quantification of the exogeneous stiffness gradient (d). Results are shown in Fig. 4c. e–l, Ectopic migration analysis. e, Schematic illustrating how the ectopic migration index (emi) was calculated. For each neural crest stream a vector was drawn from their origin to the final position of migration. The control and experimental side of the same embryos were analysed. For the control side the vector always lays in the migratory pathway, while for the experimental side some vectors point to ectopic locations. f, The difference between these two vectors \(\mathop{{\rm{a}}}\limits^{\rightharpoonup },\mathop{{\rm{b}}}\limits^{\rightharpoonup }\) generates the ectopic migration, which normalized by the control vector corresponds to the emi, which is shown as an scalar value in Fig. 4d. g, h, emi vectors for the experiment described in Fig. 4c. i–l, In situ hybridization for Twist of control and experimental side of embryos treated with exogenous local pressure (i, j) or an SDF1 bead (k, l) and the associated vectors. Scale bar is 250 μm (i, k). Thick bars (b, d) represent mean; error bars (b, d) represent s.d.; Tukey’s test (b, control; d), Dunn’s test (b, ablation); ns, P>0.05, *P≤0.05, ***P≤0.001, ****P≤0.0001; n = 11 (b, control), 10 (b, ablation), 12 (d), 9 (g, h, j, l) embryos. Diagrams in a are adapted from Normal table of Xenopus laevis (Daudin). Copyright © 1994, Nieuwkoop and Faber. Reproduced by permission of Taylor and Francis books US. Statistics and reproducibility are in the source data and Methods
