Extended Data Fig. 3: The neural crest self-generates the stiffness gradient through N-Cadherin.
From: Collective durotaxis along a self-generated stiffness gradient in vivo
a, b, Schematics indicating the different treatments (a) and embryos stained by in situ hybridization for the neural crest marker, Slug (b). Heat maps of this experiments are shown in Fig. 1f. Scale bar is 200 μm (b). c, Embryos stained by in situ hybridization for the neural crest marker, Slug. Black boxes represent the region in which nanoindentation was performed; mean stiffness heat map of each condition is shown in Fig. 1h. Scale bar is 200 μm (c). d, Diagram illustrating “chase and run”12. Neural crest (pink) chemotax toward Sdf1-secreting placode (Sdf1, purple; placode, blue). The two cell types interact through N-Cadherin (green), causing placode to run away (migration indicated by black arrow). e, Diagram illustrating placode cultured on fibronectin (grey) or fibronectin with N-Cadherin. Placodal stiffness measured in these conditions is shown in Fig. 1i. f, Schematics indicating the different treatments. Results are shown in Fig. 1l–n. g, h, exogenous stiffness gradient formation. Stiffness heat map from a representative embryo in which an exogeneous local pressure was applied ventral to the neural crest as depicted in g (bottom of the heat map, g), and quantification along the axis (h). Thick lines (h) represent mean; error bars (h) represent s.d.; Dunn’s test (h); *P≤0.05, **P≤0.01; n = 4 (h) linear lines. Diagrams in a, f are adapted from Normal table of Xenopus laevis (Daudin). Copyright © 1994, Nieuwkoop and Faber. Reproduced by permission of Taylor and Francis books US. Statistics and reproducibility are in the source data and Methods
