Extended Data Fig. 7: Structural features of SGLT1 and substrate selectivity.
From: Structure and mechanism of the SGLT family of glucose transporters
a, The density map of the cholesteryl hemisuccinate binding site of SGLT1conHA. The cholesteryl hemisuccinate density map is indicated by dashed red oval. b, Structural overlay of SGLT1conHA (orange) and a dopamine transporter DAT (cyan, PDB: 4M48). The helices near the cholesterol binding site are shown as ribbons. c, The overall organization of the lid domain of SGLT1con. Disulfide bonds are indicated by dashed blue circles. d, Close view of the four disulfide bonds. e, For five simulations of cholesterol-bound SGLT1con, the heavy atom RMSD of cholesterol from its initial position is plotted over time. f, Conservation surface mapping of SGLT1. The conservation scores are calculated from 200 SGLT1 sequences using ConSurf. The cytosolic vestibule (middle) and extracellular cavity (right) are indicated by dashed yellow oval. g, Uptake activities of SGLT1 T287A variant (mean ± SEM; n=3 biological replicates. WT and control are the same as in Fig. 2c). h, αMDG (left) and galactose (right) uptake activities of SGLT1 variants with point mutations in the substrate binding pocket on residues that are different between SGLT1 and vSGLT (mean ± SEM; n=3 biological replicates).
