Extended Data Fig. 6: Functional characterization, cryo-EM sample preparation, and data processing of SMCT1.
From: Structure and mechanism of the SGLT family of glucose transporters
a, Time course of pyruvate uptake by SMCT1 expressing oocytes (mean ± SEM; n=3 biological replicates). b, The transport of pyruvate by SMCT1 in the presence of various concentrations of pyruvate (mean ± SEM; n=3 biological replicates). c, The size-exclusion chromatography of SMCT1-Nb2 complex and SDS-PAGE analysis of the purified sample. Data are representative of five independent experiments with similar results. d, Representative cryo-EM micrograph of the SMCT1-Nb2 complex (from 8,823 micrographs with similar results). e, Selected 2D class averages of SMCT1-Nb2 complex particles (from 100 classes with similar results). f, The workflow of data processing on the SMCT1-Nb2 complex. The overall nominal resolutions of the SMCT1-Nb2 complex were determined by the ‘gold standard’ FSC curve using the FSC=0.143 criterion.
