Extended Data Fig. 5: Normalization of slide-DNA-seq sequencing biases.
From: Spatial genomics enables multi-modal study of clonal heterogeneity in tissues
a, Top, raw sequencing reads per 1 Mb bin for mouse cerebellum slide-DNA-seq are plotted for GC-content, mappability, replication timing score, and Tn5 bias. Pearson’s r values are shown for each. Bottom, bias corrected coverage and correlation values after normalization. b, Same as a but for tagmentation-based bulk sequencing of mouse cerebellum (Methods). c, Same as a but for slide-DNA seq of mouse liver metastases. d, Same as b but for tagmentation-based bulk sequencing of mouse liver metastases. Blue points, bins from chrX (not included in the calculation of the fit).
