Extended Data Fig. 6: IFNγ-responsive skin fibroblasts are required for driving vitiligo pathogenesis.
From: Anatomically distinct fibroblast subsets determine skin autoimmune patterns
a, Schematic diagram and representative FACS profiles of skin cells isolation. Detailed gating strategies are described in method section. QPCR analyses of cell-type-specific signature genes in FACS-isolated populations include KRT14 for keratinocytes, DCT for melanocytes, CD45 for immune cells, PDGFRA for fibroblasts, and CD31 for endothelial cells respectively. QPCR validation of IFNGR1 knockout used FACS-purified endothelial cells, keratinocytes, immune cells, melanocytes, fibroblasts and CD8+ T cells from TekCre;IFNGR1fl/fl, K14Cre;IFNGR1fl/fl, Csf1rCre;IFNGR1fl/fl, TyrCreER;IFNGR1fl/fl, CD4Cre;IFNGR1fl/fl, and PdgfraCreER;IFNGR1fl/fl mice, all with WT mice as controls. b, Representative whole-mount immunofluorescent staining images of melanocytes and CD8+ T cells in control and six cell-type-specific conditional knockout lines at Day 33 after vitiligo induction. c, Quantification of skin CD8+ T cells in WT and six cell-type-specific knockout lines at Day 33 after vitiligo induction procedure based on wholemount staining. d, e, Representative FACS profiles (d) and quantification (e) of CD117+ epidermal melanocytes and CD3+CD8+ T cells in control and six cell-type-specific conditional knockout lines post vitiligo induction. f, Schematic diagram of melanoma/Treg-induced vitiligo procedure and representative tail skin images (Day 60) of WT and PdgfraCreER;IFNGR1fl/fl cKO mice after vitiligo induction. Vit. induc.: Vitiligo induction. Scale bars, 500 µm (b, f). For exact p values, see Source Data. For statistics, p summary and sample sizes, see Methods.
