Extended Data Fig. 9: Anatomically distinct dermal fibroblasts have intrinsic differences in the IFNγ response.

a, Heat map of upregulated genes in human dermal fibroblasts from different anatomical regions after IFNγ treatment (> 2-fold, t test p < 0.01). b, HOX expression pattern (based on RNA-seq results) in human dermal fibroblasts from eight different anatomic regions with or without IFNγ treatment during in vitro culture. c, QPCR analysis of HOXB8, HOXC8, HOXB13, and HOXD11 in human dermal fibroblasts from eight anatomic regions. d, JAK1, JAK2, IFNGR1, and STAT1 expression pattern (based on RNA-seq results) in human dermal fibroblasts from eight different anatomic regions with or without IFNγ treatment. e, QPCR analysis of TNFSF10, IL6, and CTSH in human dermal fibroblasts from eight anatomically distinct regions after IFNγ treatment. f, Left, representative whole-mount images and quantification of hair follicle density in the dorsal and paw dorsal skin of mice at P21. Right, representative section image and quantification of skin thickness in the dorsal and paw dorsal skin of mice at P60. g, Representative immunofluorescent images, and quantification of hair follicle located melanocytes in ventral and paw ventral skin of control mice and vitiligo mice (at Day 300). h, FACS analysis and quantification of skin CD3+CD8+ T cells and CD117+ melanocytes from paw dorsal, paw ventral, dorsal, and ventral regions at Day 300 after vitiligo induction. i, Schematic diagram of fibroblast isolation and qPCR analysis of CXCL9 and CXCL10 in fibroblasts from four distinct regions of long-term vitiligo mice. j, Quantification of migration ratio using medium without fibroblast, corresponding to Fig. 4g. k, Relationship between melanocyte number and infiltrated CD8+ T cell number in each scale after vitiligo induction based on quantification data in Extended Data Fig. 4h. Scale bars, 100 µm (f), 50 μm (g). For exact p values, see Source Data. For statistics, p summary and sample sizes, see Methods.

Source data