Extended Data Fig. 1: Comprehensive analysis of different cell populations from patients with vitiligo and healthy donors.
From: Anatomically distinct fibroblast subsets determine skin autoimmune patterns
a, Representative images of the skin of patients with vitiligo showing characteristic bilateral symmetric lesion patterns in different body regions. b, Experimental workflow and representative FACS profiles to obtain single cells from human skin biopsies for single cell RNA-seq. c, Clinical information for all donors with the number of cells sequenced in single-cell analysis. d, t-SNE visualization of all collected cells showing eight main cell types with distinct clusters. e, Heat map analysis of differentially expressed genes in each cell type. f–h, violin plot (f), feature plot (g), and dot plot (h) analysis of signature genes for each cell type. i, Volcano plot and GO analysis of genes enriched in melanocytes from the M1 cluster compared to M2 cluster. j, T-SNE projection of T cells from patients with vitiligo and healthy donors. k, Volcano plot showing genes differentially expressed (>2-fold, t test p < 0.01) in each T cell sub-cluster. l, m, Representative immunofluorescent images (l) and quantification (m) of T cell subtype markers GZMA, ZNF683, CD40LG, and FOXP3 in T cells (CD3+) in the skin of patients with vitiligo. n, GO categories of genes enriched in each cell type from the skin of patients in the progressive state compared to healthy donor skin. o, Heat map analysis of 20 IFNγ response genes in eight cell types from the skin of patients in the progressive state and healthy donor skin. p, Violin plot showing the expression of IFNG in T cell subtypes. q, Violin plots showing IFNGR1 and IFNGR2 expression in different skin cell types. r, Representative immunofluorescent staining images, quantification (left and middle), and regression analysis (right) between density of CD8+ T cell and pSTAT1+ cell in the skin of patients with vitiligo. s, Representative immunofluorescent staining images of pSTAT1 signal in each cell type in the skin of patients with vitiligo. Scale bars, 50 µm (l, r, s). For exact p values, see Source Data. For statistics, p summary and sample sizes, see Methods.
