Extended Data Fig. 4: Analysis of wild-type and Ifngr1 KO mice with three different vitiligo induction methods.

a–j, Analysis of WT and Ifngr1 KO mice with melanoma/Treg-induced vitiligo model. a, Schematic diagram. b, Representative tail skin images of WT and Ifngr1 KO mice at Day 60 after vitiligo induction. c, d, Representative whole-mount images (c) and quantification (d) of tail skin epidermal melanocytes and CD8+ T cells at Day 33 after vitiligo induction. e, f, Representative FACS profile of CD117+ melanocytes and CD3+CD8+ T cells (e), and quantification of CD117+ melanocytes (f) in the tail skin epidermis at Day 33 after vitiligo induction. g, Whole-mount views of scales in tail skin epidermis (dotted line) in control and vitiligo mice. This is the unit area we used to quantify melanocyte or CD8+ T cell density in wholemount analysis throughout this paper. h. Scatter plots and correlation analysis of melanocyte number versus CD8+ T cell number in each scale of WT and Ifngr1 KO tail skin at Day 33 after vitiligo induction. i, Representative whole-mount images of DCT+ melanocytes in tail skin epidermis of WT and Ifngr1 KO mice at 4 months after vitiligo induction. j, QPCR analysis of GZMA, GZMB, PRF1, and CCL5 expression in skin CD8+ T cells isolated from WT and Ifngr1 KO mice at Day 33 after vitiligo induction. k–p, Analysis of WT and Ifngr1 KO mice with Pmel transgenic spontaneous vitiligo mouse model. k, Schematic diagram. l–m, Representative tail skin images (l, P70) and whole-mount immunofluorescent staining images (m, P42) of Pmel;WT and Pmel; Ifngr1 KO mice. n, o, Representative FACS profiles (n) and quantification (o) of CD117+ melanocytes and CD45+CD3+CD8+ T cells in tail skin epidermis of WT, Ifngr1 KO, Pmel;WT and Pmel;Ifngr1 KO mice at P42. p, Scatter plots and correlation analysis of melanocyte number versus CD8+ T cell number in each scale in Pmel;WT and Pmel;Ifngr1 KO tail skin at P42. q–x, Analysis of WT and Ifngr1 KO mice with adoptive T cell transfer-based vitiligo mouse model. q, Schematic diagram. r, Quantification of CD117+ melanocytes and CD45+CD3+CD8+ T cells in tail skin epidermis of WT mice after γ irradiation, with or without WT Pmel T cell transfer or AVV-hPMEL intraperitoneal injection. These data indicate sub-lethal irradiation alone, irradiation with hPMEL-AAV alone, or irradiation with Pmel CD8+ T cell transfer alone did not result in melanocyte loss or CD8+ T cell infiltration in WT tail skin epidermis. s, Representative immunofluorescent staining images and FACS quantification of epidermal melanocyte of mice post γ-irradiation 26 days with controls, indicating after 5 Gy γ irradiation leads to ~2-fold increase of melanocyte number in tail skin epidermis compared to untreated mice. t, Representative FACS profiles and quantification of CD3+CD8+VB13+ Pmel T cells in spleen or skin of WT and Ifngr1 KO mice at Day 26 with or without adoptive transfer-based vitiligo model induction. These data showed although both WT and Ifngr1 KO host mice contained the same number of transferred WT TCR VB13+ Pmel T cells in the spleen, only WT host mice exhibit robust VB13+ Pmel T cell infiltration in skin, whereas the Ifngr1 KO host mice did not. u–v, Representative tail skin images (u, Day 60) and whole-mount immunofluorescent images (v, Day 26) of WT and Ifngr1 KO mice post vitiligo model-induction. Scale bars, 500 µm. w, Representative FACS profiles and quantification of epidermal CD117+ melanocytes in WT and Ifngr1 KO mice at Day 26 post vitiligo model-induction. x, Scatter plots and correlation analysis of melanocyte number versus CD8+ T cell number in each scale in WT and Ifngr1 KO mice at Day 26 post vitiligo model-induction. Scale bars, 500 µm (b, c, g, i, l, m, s, u, v). For exact p values, see Source Data. For statistics, p summary and sample sizes, see Methods.

Source data