Extended Data Fig. 7: Additional information about mutations in miR-431-5p, miR-140-3p and miR-677-5p.

a) Table depicting the name and sequence of the CELLmotif AGAAC incorporated in miR-431-5p. Bold underlying text in the sequence indicated changed nucleotides in the guide strand of the miRNA used to introduce the CELLmotif AGAAC. Nucleotides in the passenger strand were also modified to maintain miRNA structure. b) Predicted structure for the hairpin miRNA for the constructs shown in a. Red means high probability of pairing, while blue indicates low probability calculated by RNAfold WebServer software. Arrow indicates the location of the mutated nucleotides. c) Normalized gene expression for miR-431-5p wild-type in cells and sEV for each cell type. Average Ct from the whole miRNA profile was used for normalization for each sample. d) Table depicting the name and sequence of the wild-type miR-140-3p and mutated version without CELLmotif AGAAC. Bold underlying text in the sequence indicated changed nucleotides in the guide strand of the miRNA used to remove the CELLmotif AGAAC. Nucleotides in the passenger strand were also modified to maintain miRNA structure. e) Predicted structure for the hairpin miRNA for the constructs shown in d. Red means high probability of pairing, while blue indicates low probability. Arrow indicates the location of the mutated nucleotides. f) Normalized gene expression for miR-140-3p wild-type in cells and sEV for each cell type. Average Ct from the whole miRNA profile was used for normalization for each sample. g) Table showing the name and sequence of the wild-type miR-677-5p and the version in which CELLmotifs were mutated. Bold underlined text in the sequence indicated mutated nucleotides in the guide strand of the miRNA to remove CELLmotifs CAGU and AUU[A/G]. Nucleotides in the passenger strand were also modified accordingly to maintain miRNA structure. h) Predicted structure for the hairpin miRNA for the constructs shown in g. Red means high probability of pairing while blue indicates low probability. Arrows indicate the location of the mutated nucleotides. i) Normalized gene expression for miR-677-5p wild-type in cells and exosomes/sEV for each cell type. Average Ct from the whole miRNA profile was used for normalization for each sample. Data are expressed as mean ± SEM. n=3-4. *P≤0.05 (Limma t-test).