Extended Data Fig. 5: Comparison of miRNA profiling of AML12 and primary hepatocytes.

Primary hepatocytes were isolated from C57Bl/6J wild-type mice (n=4) and cultured for 48 h in exosome-free medium to collect sEV and cell lysates, which were later subjected to RNA isolation and miRNA profiling for comparison to AML12 hepatocytes. a) Number of miRNAs showing selective cellular retention, non-selective distribution or selective sEV sorting in AML12 and primary hepatocytes. The selective distribution column is the sum of sEV- and cell-enriched miRNAs divided by the total number of miRNAs detected as percentage. FDR < 0.1. b) Venn-diagram showing the number of sEV- (red) and cell-enriched miRNAs (blue) in AML12 and primary hepatocytes and the overlap between them. c, d) Motifs associated to sEV (c) and cell (d) enrichment in primary hepatocytes. The table shows the significance of the enrichment (P-value), false discovery rate (FDR), the percentage of miRNAs significantly enriched in sEV (in c) or cell (in d) that contain the motif, the percentage of miRNAs not enriched in the background miRNAs containing the motif and the fold-enrichment as the ratio between the previous two columns.