Extended Data Fig. 2: Resolution estimates for cryo-EM reconstructions, atomic B-factor refinement and model-map FSC.

a, Local resolution estimates for the GR-loading complex global reconstruction were calculated using RELION with front view (left) and back view (right). b, Euler angle distribution in the final reconstruction. Orthogonal views of the reconstruction are shown with front view (top) and side view (bottom) c, Gold-standard FSC for the global cryo-EM reconstruction. d, Atomic model with B-factors refined with colour key shown on the left. e, Histogram of the B-factor values of all non-hydrogen atoms in the atomic model, coloured by the same colour key in d. f, Model-map FSC. g, Focused classification and refinement of the GR-loading complex. Masks were created at various regions of the GR-loading complex (left) and its corresponding gold-standard FSC (right) after 3D auto-refine. The nominal resolution for each reconstruction is labelled and indicated in the FSC plots.