Fig. 4: Replication of Alpha, wt-SAlpha, and wt-S614G in hACE2-K18Tg mice.
From: Enhanced fitness of SARS-CoV-2 variant of concern Alpha but not Beta
a, b, Two groups of four donor hACE2-K18Tg mice were inoculated with 1 × 104 PFU, determined by back titration and comprising a mixture of wt-S614G (orange) and Alpha (dark blue) at a 3:1 ratio (a), or a mixture of wt-S614G and wt-SAlpha (light blue) at a 1:1 ratio (b). Pie charts show the ratio of variants detected in each sample at the indicated dpi. Pie chart sizes are proportional to the total number of viral genome copies per ml (swabs) or per sample (tissues), as shown below each chart. Grey pies indicate values below the LOD. Mouse silhouettes are coloured to indicate the dominant SARS-CoV-2 variant (>66%) in the last positive swab sample from the corresponding mouse; a silhouette with two colours indicates that there is no dominant variant. K18 nos. 1 to 8 denote individual hACE-K18Tg donor mice.
