Extended Data Fig. 1: ACE2 receptor binding and replication kinetics of SARS-CoV-2 VOC in vitro.
From: Enhanced fitness of SARS-CoV-2 variant of concern Alpha but not Beta
(a) Affinity between spike (S614G, SAlpha, and SBeta) protein trimers and hACE2 dimers determined by Bio-layer interferometry. (b) Viral replication kinetics of SARS-CoV-2 Alpha, Beta, and wt-S614G (MOI 0.02) at 33 °C and 37 °C in primary human nasal airway epithelial cell (AEC) cultures. (c) Viral replication kinetics of pairwise competition assays in primary nasal AEC cultures at 33 °C (MOI 0.005). (b, c) Data are presented as individual points with mean (line) and standard deviation; (b) n = 2 (Alpha and Beta), n = 4 (wt-S614G), (c) n = 3 independent biological replicates. (c) P-values were determined by two-way ANOVA and Tukey Honest Significant Differences (HSD) post-hoc test. (d) Affinity between spike (S614G, SAlpha) protein trimers with hamster ACE2 determined by Bio-layer interferometry. (a, d) ACE2 with IgG1 Fc tag were loaded on anti-human IgG Fc biosensors and binding kinetics were conducted using indicated concentrations of spike trimers. Data is representative of 3 independent experiments.
