Extended Data Fig. 5: Infection with the SARS-CoV-2 Alpha variant results in lower IFN III and pro-inflammatory responses than first wave isolates.
From: Evolution of enhanced innate immune evasion by SARS-CoV-2
a, Calu-3 cells were infected with 250 E copies/cell and IFNL1 and IFNL3 expression measured at 24 hpi. b, Secretion of CXCL10, IL6 and CCL5 by infected cells at 48 hpi. c, d, Calu-3 cells were infected with (c) 5,000 E copies/cell or (d) 5 E copies/cell. Expression of TNF, CCL2, IL6, IL8 and CCL3 were measured. Data shown are mean ± s.e.m. of one of three representative experiments performed in triplicate. One Way ANOVA with a Tukey post-comparison test (a, b) or two Way ANOVA (c,d) were used. Blue stars indicate comparison between Alpha and VIC (blue lines and symbols), grey stars indicate comparison between Alpha and IC19 (grey lines and symbols). * (p < 0.05), ** (p < 0.01), *** (p < 0.001), **** (p < 0.0001). ns: non-significant. E: viral envelope gene.
