Extended Data Fig. 11: Convergent differential expressed genes for the three mutations.
From: Autism genes converge on asynchronous development of shared neuron classes
a, Log fold change of all genes which showed significant change (adjusted p < 0.05) in all three of the 1 month datasets: Mito210 SUV420H1+/− 35 d.i.v., Mito210 ARID1B+/− 35 d.i.v., and HUES66 CHD8+/− 35 d.i.v. DEGs were calculated using all cells as a pseudobulk for Mito210 SUV420H1+/− and Mito210 ARID1B+/−. b, Differential expression of all 102 genes associated with ASD risk6 in the three datasets Mito210 SUV420H1+/− 35 d.i.v., Mito210 ARID1B+/− 35 d.i.v. and in HUES66 CHD8+/− 35 d.i.v. compared to relative controls. Expression of risk genes was calculated using all cells (pseudobulk) for Mito210 SUV420H1+/− and Mito210 ARID1B+/−. Boxes are coloured according to -log10(adjusted p value) according to whether they are upregulated (purple), or downregulated (turquoise) in mutant vs. control. Genes are ordered according to hierarchical clustering (using Euclidean distance) of those values. c, d, Enriched GO terms for genes upregulated (c) and downregulated (d) in mutant vs. control. Genes were calculated using the cells as in a, b. The top 5 most significant terms per dataset are shown. Size of dot indicates the proportion of genes belonging to each term found in the list of dysregulated genes (“GeneRatio”). Colour indicates enrichment adjusted p-value. Numbers in parentheses along the x axis indicate the number of DEGs in that dataset.
