Extended Data Fig. 4: Validation of additional hits identified from CRISPR screen of SpeB-triggered lytic cell death.
From: Streptococcal pyrogenic exotoxin B cleaves GSDMA and triggers pyroptosis
a, List of top hits from CRISPR screen of SpeB-triggered lytic cell death. b, GSDMA-knockout cells used in this study. Gene coding sequences were present as black boxes. Top sequence track is the gene wild-type allele. Location of gRNAs is indicated with blue bars and PAM sequences were underscored. c–l, WT and the indicated gene knockout A431 cells were transfected or not with recombinant SpeB by electroporation, followed by cell morphology observation by phase-contrast microscopy (c, e, g, i, k), cell viability assessment by CellTiter-Glo luminescent assay (d, f, h, j, l). Scale bar: 10 μm. Graphs show mean ± s.d. of triplicate wells. Data are representative of at least three independent experiments.
