Extended Data Fig. 3: Lack of inflammatory signals in peripheral and brain cytokine and immune profiles of 4EP+/− mice.
From: A gut-derived metabolite alters brain activity and anxiety behaviour in mice
a, b, Cytokine and chemokine levels presented in bar graph where grey (−) is 4EP− and green (+) is 4EP+, measured by bioplex in colon (n=9) a, and serum (n=9) b. No significant differences were observed. c–d, Flow cytometry of spleens of 4EP+ and 4EP− mice. For gating strategy see Supplementary Fig. 2. c, Percentages of CD4+ or CD8+ T cells (4EP− n=11, 4EP+ n=10). d, Percentage of B cells (4EP− n=11, 4EP+ n=10)p<0.0001. e, Cytokine and chemokine levels presented in bar graph where grey (−) is 4EP− and green (+) is 4EP+, measured by bioplex (n=9) in brain tissue (Il-3 p=0.046; Il-5 p=0.01; KC p=0.02; TNF-a p=0.01). f, Flow cytometry percentages of microglia in the brain of 4EP− and 4EP+ mice. For gating strategy see Supplementary Fig. 2. g, Relative expression of microglial genes in microglial-enriched samples (n=8). Multiple randomized litters were used for the experiments in the figure. Each data point represents biologically independent mice from multiple randomized litters examined over one (a-b) or two (c-h) respective experiments. Data represent mean ± SEM. Panels a, b, d, f, and h were analysed by one-way ANOVA with Dunnett multiple comparisons test comparing each 4EP+/4EP− pair, and panels g and e by a two-tailed Welch’s t-test. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001.
