Extended Data Fig. 7: Recurrent mutagenesis and functional effects of kyklonas.
From: Mapping clustered mutations in cancer reveals APOBEC3 mutagenesis of ecDNA
a, The total number of recurrently mutated ecDNA displayed as a proportion of the total number of ecDNA with kyklonas for a given cancer type. The total number of ecDNA with kyklonas are displayed above each bar plot for each cancer type. All ecDNA with recurrent hypermutation were considered enriched for kyklonic events after correcting for multiple hypothesis testing (Z-score test; q-values < 0.05). b, Proportion of samples containing ecDNA divided exclusively into those with co-occurring kataegis, no kataegis overlap, and no detected kataegis across the entire genome. The number of samples included in each cancer type are listed. For certain cancer types, as few as a single sample may represent the entire proportional breakdown (for example, Bone-Osteosarc or Bone-Epith). c, A single sarcoma genome and d, a single head squamous cell carcinoma genome depicting the overlap of kataegis with ecDNA regions displayed as a rainfall (top left) with a single zoomed in ecDNA represented using a circos plot (top right). Bottom: Two regions of the ecDNA with overlapping kyklonic events. VAFs are shown per event (orange). e, Kyklonic substitutions resulting in recurrent coding mutations within known cancer genes.
