Extended Data Fig. 5: Trpγ+ neurons are a diverse population.
From: A discrete neuronal population coordinates brain-wide developmental activity
a–i. Top: 13µm-thick (A-C) or full (D-I) MIPs of 72 hAPF brains stained for neuronal class marker (yellow), Trpγ+ nuclei (mCherry-NLS or GFP-NLS, cyan), and a reference marker (Ncad, magenta). Images (A-C, E-H) derived from three stitched confocal stacks. Scale bar, 100µm. Bottom: Expanded view(s) of ROI(s) boxed in top panel. Columns (D, rows) are class marker, Trpγ+, and merged channels, left to right (D, top to bottom). Marked Trpγ+ nuclei (red asterisks) co-localize with the neuronal class marker. ROI 2 in (G) shows transient PDF-TRI cells (38). Scale bar, 20µm.
