Extended Data Fig. 7: Additional characterization of Trpγ+ neuron activity.
From: A discrete neuronal population coordinates brain-wide developmental activity
a. Representative autocorrelograms from pan-neuronal GCaMP6s in control (empty-GAL4, black), panN-GAL4>Kir2.1 (blue), and TrpγG4>Kir2.1 (orange) pupae. b, c. Representative micrographs (B) and traces (C) from 2PM imaging of pan-neuronal GCaMP6s in control (empty-GAL4, black), panN-GAL4>Kir2.1 (blue), and TrpγG4>Kir2.1 (orange) pupae. Scale bar, 40µm. d. Inset: expanded view showing fewer sweeps in panN-GAL4 and TrpγG4 conditions. e. Representative traces for Trpγ+ neurons expressing GCaMP6s (cyan, n=10) and pan-neuronal expression of GCaMP6s (black, n=10) by wide-field imaging with a ROI encompassing the head. f. Active phase average amplitude for Trpγ+ neurons expressing GCaMP6s (cyan) binned by hour and normalized to pan-neuronal expression of GCaMP6s (black). Shaded areas, SD. g, h. AIP of pupae expressing pan-neuronal GCaMP6s (g). ROIs indicate regions used to calculate traces (h) from optic lobes. Scale bar, 200µm. i. 0-lag correlation between traces in each optic lobe in control (empty-GAL4, black, n=4), and TrpγG4>Kir2.1 (orange, n=4) pupae. Round markers are values from individual time series, bars are averages for each genotype. j. Correlogram between traces in each optic lobe in TrpγG4>Kir2.1 pupa. k. Cell-type-specific Brp puncta counts in control (empty-GAL4>Kir2.1 pupae, black, n=35 per cell type), PanN-GAL4>Kir2.1 pupae (cyan, n=40 for Dm9, n=25 for Tm9) and in TrpγG4>Kir2.1 pupae (orange, n=40 cells for Dm9, n=35 cells for Tm9).
