Extended Data Fig. 9: Trpγ+ neurons are necessary for PSINA.
From: A discrete neuronal population coordinates brain-wide developmental activity
a. Temporal expression control with TARGET; animals shifted from 18oC to 29oC at 40 hAPF. b. PSINA traces from pan-neuronal GCaMP6s in control (empty-GAL4, black, n=3), panN-GAL4>hid, rpr (blue, n=3), and TrpγG4>hid, rpr (orange, n=3) pupae. c. Average amplitude normalized to control activity between 55 and 75 hAPF. Shaded areas, SD. Genotypes color-matched to B. d. PSINA traces from pan-neuronal GCaMP6s in control (empty-GAL4, black, n=7), panN-GAL4>TNT (blue, n=7), and TrpγG4>TNT (orange, n=8) pupae. e. Average amplitude normalized to control activity between 55 and 75 hAPF. Shaded areas, SD. Genotypes color-matched to D. f. Representative traces of pupae expressing pan-neuronal GCaMP6s, with TNT expressed in expression domains of the indicated neuronal class. n=4 tested for each genotype.
