Extended Data Fig. 2: Locomotion of male flies.
From: A nutrient-specific gut hormone arbitrates between courtship and feeding
(a–c) Male flies exhibit similar locomotion activity after consuming food containing isocaloric sucrose or amino acids. After deprived of food for 24 h, individual flies were given access to food for 10 min. Male flies were then placed in a circular arena (60 mm in diameter, 3.5 in mm depth) for video recordings. Food: sucrose (50 mM) or amino acids (200 mM). (a) Walking trajectories of individual flies during the 5-min observation period. (b) Average walking velocity of individual flies. n = 10 flies for each food. Two-tailed t-test. (c) Average path length traveled by flies. n = 10 flies for each condition. (d–f) Gut Dh31 knockdown does not affect locomotion activity. Ctrl1: Dh31-GAL4, Tsh-GAL80. Ctrl2: UAS-Dh31-RNAi. Gut Dh31 KD flies contain all three transgenes. (d) Walking trajectories of individual flies. (e) Average walking velocity of individual flies during the 5-min observation period. n = 10 flies for each genotype. One-way ANOVA test. (f) Average path length traveled by flies. n = 10 flies for each genotype.
