Extended Data Fig. 3: Schematic illustration of gene structures and knock-in constructs. | Nature

Extended Data Fig. 3: Schematic illustration of gene structures and knock-in constructs.

From: A nutrient-specific gut hormone arbitrates between courtship and feeding

Extended Data Fig. 3

(a) A T2A-GAL4.2 cassette was inserted at the C-terminus of Dh31 by Cas9-mediated knock-in strategy. 3XP3-RFP was used as a positive selection marker. (b) An nls-LexA::p65 cassette was inserted after the start codon of Dh31R by Cas9-mediated knock-in strategy. (c) An nls-LexA::p65 cassette was inserted after the start codon of Crz by Cas9-mediated knock-in strategy.

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