Extended Data Fig. 4: Analysis of 15N and 1HN relaxation dispersion data of JIP1-SH3.
From: Visualizing protein breathing motions associated with aromatic ring flipping
a, Examples of 15N CPMG relaxation dispersion profiles for JIP1-SH3 obtained at two magnetic field strengths (red – 600 MHz, blue – 850 MHz) at 15 °C. b, Examples of 1HN CPMG relaxation dispersion profiles for JIP1-SH3 obtained at two magnetic field strengths (red – 600 MHz, blue – 950 MHz) at 15 °C. The 15N and 1HN data were analysed simultaneously for all residues according to a two-site exchange model (full-drawn lines in a and b). Error bars in a and b represent one standard deviation (s.d.) derived from Monte Carlo simulations of experimental uncertainty. c, d, Chemical shift differences between the major and minor state extracted from a simultaneous analysis of the relaxation dispersion data at 15 °C for 15N (c) and 1HN (d).
