Extended Data Fig. 9: Plasma reactivity against EBNA1 and GlialCAM proteins and peptides in healthy control individuals and MS patients.

a, ELISA measurement of antigen-specific IgG reactivity against peptides EBNA1_AA386-405, GlialCAM_AA370-389, phosphorylated GlialCAM_{AA370-389 pSer376}, 2x-phosphorylated GlialCAM_{AA370-389 pSer376 pSer377}, and scrambled peptide control in plasma samples of healthy control individuals (n = 50) and MS patients (n = 71). Means ± SD in each patient group is shown. Representative OD (450 nm) measurements of two independent experiments, each carried out in duplicates. **P < 0.01, ***P < 0.001 according to two-tailed Mann-Whitney test, Tukey corrected for multiple comparisons. b, ELISA measurements of antigen-specific IgG reactivity against GlialCAM full-length protein, GlialCAM_AA370-389, and phosphorylated GlialCAM_{AA370-389 pSer376} in plasma samples of a separate cohort of healthy control individuals (n = 31) and MS patients (n = 67). Means ± SD across patient groups are shown. Representative OD (450 nm) measurements of two independent experiments, each carried out in duplicates. *P < 0.05, **P < 0.01 according to two-tailed Mann-Whitney test, Tukey corrected for multiple comparisons. c, ELISA measurements of mAB MS39p2w174 binding to EBNA1_AA386-405, without interference as well as blocked with scrambled peptide control, EBNA1_AA386-405, and GlialCAM_{AA370-389 pSer376}, as a positive control to (Fig. 3q). Mean OD (450 nm) ± SD of quadruplicate measurements from n = 1 experiment are shown. *P < 0.05, **P < 0.01, ***P < 0.001 according to one-way ANOVA, Tukey corrected for multiple comparisons.

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