Extended Data Fig. 1: Analysis of B cell phenotypes in MS blood and CSF.
From: Clonally expanded B cells in multiple sclerosis bind EBV EBNA1 and GlialCAM
a–l, Flow cytometry data, a,b, representative flow cytometry plots are shown for a, blood and b, CSF. c, Plasmablasts as percent of all B cells in MS blood and CSF, means ± SD of n = 9 patient samples, **P = 0.004, two-tailed Mann-Whitney test. d, Non-plasmablast B cell subsets as percent of all B cells in blood (red) and CSF (blue), means ± SD of n = 8 patient samples, ***P = 0.0006, two-tailed Mann-Whitney test, Holm-Sidak corrected for multiple comparisons. e, Integrin alpha-4 expression in non-plasmablast B cells (red) and plasmablasts (blue), mean MFI ± SD of n = 9 patient samples, ****P < 0.0001, **P = 0.0013, two-way ANOVA, Tukey adjusted for multiple comparisons, f, representative histogram showing integrin alpha-4 expression in non-plasmablast B cells (red) and plasmablasts (blue) in blood (top panel) and CSF (lower panel), g, HLA-DR expression in non-plasmablast B cells (red) and plasmablasts (blue) in blood and CSF, mean MFI ± SD of n = 9 patient samples, ****P < 0.0001, ***P = 0.0002, two-way ANOVA, Tukey adjusted for multiple comparisons, and h, representative histogram showing HLA-DR expression in non-plasmablast B cells (red) and plasmablasts (blue) in blood (top panel), and CSF (lower panel). i, HLA-DR expression in patients carrying HLA-DRB1*15:01 (HLA-DR15, n = 5) vs. other HLA-genotypes (non-HLA-DR15, n = 4) in i, blood, and j, CSF, mean MFI ± SD, significance levels calculated with two-way ANOVA, k,l, Immunoglobulin classes in k, non-plasmablast B cells and l, plasmablasts in blood (red) and CSF (blue), mean MFI ± SD of n = 9 patient samples, ****P < 0.0001, two-way ANOVA, Holm-Sidak adjusted for multiple comparisons. Plasmablasts, PB; unswitched memory B cells, UM; switched memory B cells, SM; double negative B cells, DN.
