Extended Data Fig. 2: Western blot analysis of SARS-CoV-2 spike from virus infected VeroE6-TMPRSS2 cells.

VeroE6-TMPRSS2 cells were infected by SARS-CoV-2 WT, Delta, or Omicron at 0.01 m.o.i. Cell lysates were harvested at 48 h post infection for detection of SARS-CoV-2 spike cleavage using an anti-spike S2 antibody. Representative images of SARS-CoV-2 spike and nucleocapsid Western blots were shown with β-actin added as a sample processing control. Spike, nucleocapsid, and β-actin was run on three different gels and detected on three different membranes. The experiment was repeated three times independently with similar results.