Extended Data Fig. 4: Omicron infection in C57B6 WT mice.
From: Attenuated replication and pathogenicity of SARS-CoV-2 B.1.1.529 Omicron
6-to-8-week-old female C57B6 WT mice were intranasally inoculated with 1 × 105 PFU of Alpha or Omicron. Nasal turbinates and lungs of the infected mice were collected on 2 or 4 d.p.i. for viral burden determination (n = 8). a Viral RNA-dependent RNA polymerase (RdRp) gene copies were quantified with probe-specific RT-qPCR. b Subgenomic envelope (E) gene were quantified with probe-specific RT-qPCR. c Infectious viral titres were quantified with plaque assay in VeroE6-TMPRSS2 cells. Data represents mean ± SD from the indicated number of biological repeats. Statistical differences were determined with two-tailed Student’s t-test in (a–c). Data were obtained from two independent experiments. Each data point represents one biological repeat. * represented P < 0.05; ** represented P < 0.01; *** represented P < 0.001; **** represented P < 0.0001. ns, not statistically significant.
