Extended Data Fig. 4: Validation of differential gene expression analysis.
From: Molecular hallmarks of heterochronic parabiosis at single-cell resolution
a, Violin plots showing the number of differentially expressed genes as the function of the number of replicates per comparison. The number of replicates are defined as the minimum number of replicates within the control and treatment groups. Results are shown separately for TMS (left) and parabiosis (right). b, Comparison of differential gene expression results with and without subsampling in case of each cell type specific comparison. Spearman correlation values indicate (dis)similarities between: p-values derived from the original and subsampled data (left), effect sizes calculated from the original and subsampled data (middle), and effect sizes calculated on the original data and p-values derived from the subsampled datasets. c, Number of DEGs identified at different p-value and effect size cutoffs per comparison in AGE (left), ACC (middle), and REJ (right).
