Extended Data Fig. 4: Cryo-EM data processing of the NLRP3–CRID3 complex.
From: Structure of the NLRP3 decamer bound to the cytokine release inhibitor CRID3
a, Representative cryo-EM micrograph of the human NLRP3 decamer in complex with CRID3 (replicated more than 20,000 times). Scale bar, 50 nm. b, c, Representative 2D class averages. d, e, Processing tree of cryo-EM data in RELION3.135 as described in the Methods section and gold standard FSC plots of masked final maps (grey) as calculated from phenix-mtriage40. f–h, Orientation distribution of particles and heat maps of local resolution estimations.
