Extended Data Fig. 10: Ubiquitin and the chaperone trigger factor support lanthanide-binding tag coordination and produce substantial paramagnetic tensors.
From: Structure determination of high-energy states in a dynamic protein ensemble
(a) [1H-15N]-HSQC spectra of ubiquitin mutants S20C (left) and K6C (middle, right) bound with either the DOTA-M7PyThiazole (left, middle) or DOTA-M8-(4R4S)-SSPy(right) lanthanide-binding tags. Each spectrum shows large PCSs induced by Tm3+ bound tags. (b) Correlation plots between each ubiquitin variant’s PCSs and the calculated PCSs. (c) [1H-15N]-HSQC spectra of WT PPD-SBD, V270C PPD-SBD, M8-Lu-V270C PPD-SBD, and M8-Tm-V270C PPD-SBD. (d) Zoom-in of center section of the [1H-15N]-HSQC spectra of (c) showing nearly identical spectra for each sample. (e) Correlation plots between each M8-Tm-V270C PPD-SBD PCSs and the calculated PCSs.
