Extended Data Fig. 10: Mode of TCA cycle engagement regulates cell fate.
From: A non-canonical tricarboxylic acid cycle underlies cellular identity
a, Population doublings of control and Aco2-edited ES cells cultured in metastable (serum/LIF) conditions. b, Cumulative population doublings over the indicated passages of control and Aco2-edited ES cells upon conversion to the naive, ground state of pluripotency via addition of MEK and GSK3β inhibitors (+2i). c, RT–qPCR of pluripotency-associated genes at the indicated passages in control and Aco2-edited ES cells following addition of 2i. Gene expression at every passage was normalized to passage 0 (p0). Data are mean ± SD, n = 1 (b) or n = 3 (a, c) independent replicates. Significance was assessed in comparison to control cells by one-way ANOVA with Sidak’s multiple comparisons post-test (a) or relative to control cells at each timepoint with P values coloured according to comparison by two-way ANOVA with Sidak’s multiple comparisons post-test (c).
