Extended Data Fig. 3: Rough ER is downregulated in obesity.
From: Regulation of liver subcellular architecture controls metabolic homeostasis
a, Immunoblot analysis of the indicated proteins and validation of the rough – smooth ER fractionation. TL: total liver lysate, RER: rough endoplasmic reticulum, SER: smooth ER, Mito: mitochondria, Cyto: cytosolic fraction. b, TEM of rough and c, smooth ER vesicles isolated from livers derived from lean and obese mice. ER recovered in the denser sucrose fraction is enriched in ribosomes in both lean and obese samples (b), while the smooth ER fraction was characterized by microsomes vesicles devoid of ribosomes (c). d, milligrams of rough (***p = 0.0004) and e, smooth (p = 0.0917) ER vesicles recovered by subcellular fractionation normalized by milligram of liver. f, Ratio of abundance of rough to smooth ER vesicles (**p = 0.0014) in lean and obese mice. n = 6 per group. g, Confocal images and quantification of immunofluorescence staining for endogenous Sec61β in primary hepatocytes from lean and obese mice. n = 3 fields lean and n = 5 fields obese, representative of 3 independent hepatocyte isolations (**p = 0.0018). h, Confocal images of immunofluorescence staining for endogenous Sec61β in liver sections from lean and obese mice. Right panel: Quantification of fluorescence intensity of immunofluorescence staining for endogenous Sec61β in liver sections from lean and obese mice. n = 8 fields for lean and 7 fields for obese mice, representative of 2 mice per group (****p = 0.0001). All data (Extended Data Fig. 3d–h) are presented as mean values +/− SEM. Unpaired t-test was used for all the statistical analyses.
